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Fri, Nov 24



Integrative Approach Targeting the Vagus Nerve and the Neuroendocrine-Immune System (NEIS). 2d Ed. Part 1.

The Part 1 (3-day course) will give a unique opportunity to discover the role of the vagus nerve in the bi-directional communication between the ANS and the immune system and how applied manipulative treatment can affect the sympatho-vagal balance.

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Integrative Approach Targeting the Vagus Nerve and the Neuroendocrine-Immune System (NEIS). 2d Ed. Part 1.
Integrative Approach Targeting the Vagus Nerve and the Neuroendocrine-Immune System (NEIS). 2d Ed. Part 1.

Time & Location

Nov 24, 2023, 9:00 AM AST – Nov 26, 2023, 5:00 PM AST

Truro, 692 Prince St, Truro, NS B2N 1G6, Canada

About the event

Pascal Grolaux, BSc PT, DO, MOst, Dr (Hon) MACoPM, will offer an original, evidence-based and the most comprehensive course, given in Canada and in Europe, on the Integrative Approach Targeting the Vagus Nerve and the Neuroendocrine-immune System (NEIS), including Heart Rate Variability analysis based on short-term EKG. Indeed we will integrate evidence-based applied osteopathic treatment (general and specific) and non-invasive vagus nerve and trigeminal nerve stimulation and their effects on the sympatho-vagal balance in order to offer a better global patient-centered management especially related to complex chronic diseases.


The first part of the course will give an overview of general principles of neurophysiology, neuroanatomy of the central and autonomic nervous system, neuroendocrine-immunology, general principles of inflammation and diseases, the brain-gut, brain-heart, and gut-lungs axis connexions. We will review the osteopathic centers, the principle of inhibition, general and specific osteopathic-orientated treatment to target the neuroendocrine-immune system and the ANS.

Principles of Heart Rate Variability analysis, what it is, why and how to use it in daily clinical practice, based on short-term EKG analysis, demonstration.

At the end of the course (Part 1) the participant will be able to:

  • Understand the global function of the Autonomic Nervous System with emphasis on the vagus nerve.
  • Understand the link between the vagus nerve and its connexions to the neuroendocrine-immune system.
  • Understand the principles of neuroendocrine-immunology in relation with the innate immune system and the anti-inflammatory reflexes
  • Understand the role of the central autonomic network (CAN) and the brain structures and functions linked to it.
  • Understand the common causes of disease in relation to the vagus nerve.
  • Understand the importance of the Brain-Gut, Brain-Heart and Gut-Lung’s axis in the modulation of neuro-immune reflexes.
  • Understand the osteopathic centers, the principles of the general and specific osteopathic-orientated treatment to target the neuroendocrine-immune system and the ANS.
  • Understand the principles of short-term Heart Rate Variability (HRV) analysis used as a possible biomarker of the sympathovagal balance, and how to perform it via a 3-lead ECG.
  • To conduct a general and specific-orientated treatment to influence the neuroendocrine immune system and the ANS as demonstrated by short-term HRV analysis.


·  Review of the anatomophysiology of the autonomic nervous system (ANS)

·  The limbic system

·  The central autonomic network (CAN)

·  Neuroendocrine-immunology (NEI)

·  Heart-brain axis

·  Gut-Brain axis

·  Gut-Lung’s axis

·  Common mechanisms of diseases in relation with the vagus nerve

·  Philosophy, principles, and evidence behind osteopathic treatment approach targeting the NEI and the ANS

·  The osteopathic centers and the principle of inhibition

·  Applied general and specific osteopathic approaches/techniques

·  Principles and demonstration of Heart Rate Variability Analysis based on short-term EKG

Who Should Attend This Course

This course is designed for Osteopaths, Physiotherapists, Naturopaths, med. Doctors, other Health Practitioners (if you are not sure this course is for you, please contact us).

The course will be given at Legge Clinic, in Truro, Nova Scotia.

CEUs EARNED: 21h-equivalent at the end of part 1 with a certificate issued at the end of the course.

Early birds enrolling up before Sept. 30th : use the coupon code: NEISX12d to get a 15% discount!

Note: language will be English with French translation if needed.

·  Cancellation deadline: 4 weeks before

·  Prerequisite: review of the neuroanatomy of the autonomic nervous system


Tracey, K. J. (2002). "The inflammatory reflex." Nature 420(6917): 853-859.

Hosoi, T. and Y. Nomura (2004). "Functional role of acetylcholine in the immune system." Frontiers in Bioscience 9: 2414-2419.

Rosas-Ballina, M., et al. (2008). "Splenic nerve is required for cholinergic antiinflammatory pathway control of TNF in endotoxemia." Proceedings of the National Academy of Sciences of the United States of America 105(31): 11008-11013.

d.  Sundman, E. and P. S. Olofsson (2014). "Neural control of the immune system." Advances in Physiology Education 38(2): 135-139.

Chavan, S. S. and K. J. Tracey (2017). "Essential Neuroscience in Immunology." Journal of Immunology 198(9): 3389-3397.

Henley CE, Ivins D, Mills M, Wen FK, Benjamin BA. Osteopathic manipulative treatment and its relationship to autonomic nervous system activity as demonstrated by heart rate variability: a repeated measures study. Osteopath Med Prim Care. 2008 Jun 5;2:7. doi: 10.1186/1750-4732-2-7. PMID: 18534024; PMCID: PMC2442110.

Carnevali L, Lombardi L, Fornari M, Sgoifo A. Exploring the Effects of Osteopathic Manipulative Treatment on Autonomic Function Through the Lens of Heart Rate Variability. Front Neurosci. 2020 Oct 7;14:579365. doi: 10.3389/fnins.2020.579365. PMID: 33117124; PMCID: PMC7575744

Ruffini N, D'Alessandro G, Mariani N, Pollastrelli A, Cardinali L, Cerritelli F. Variations of high frequency parameter of heart rate variability following osteopathic manipulative treatment in healthy subjects compared to control group and sham therapy: randomized controlled trial. Front Neurosci. 2015 Aug 4;9:272. doi: 10.3389/fnins.2015.00272. PMID: 26300719; PMCID: PMC4523739.

Fornari M, Carnevali L, Sgoifo A. Single Osteopathic Manipulative Therapy Session Dampens Acute Autonomic and Neuroendocrine Responses to Mental Stress in Healthy Male Participants. J Am Osteopath Assoc. 2017 Sep 1;117(9):559-567. doi: 10.7556/jaoa.2017.110. PMID: 28846122.

Cerritelli F, Chiacchiaretta P, Gambi F, Saggini R, Perrucci MG, Ferretti A. Osteopathy modulates brain-heart interaction in chronic pain patients: an ASL study. Sci Rep. 2021 Feb 25;11(1):4556. doi: 10.1038/s41598-021-83893-8. PMID: 33633195; PMCID: PMC7907192.

Żurowska A, Malak R, Kołcz-Trzęsicka A, Samborski W, Paprocka-Borowicz M. Compression of the Fourth Ventricle Using a Craniosacral Osteopathic Technique: A Systematic Review of the Clinical Evidence. Evid Based Complement Alternat Med. 2017;2017:2974962. doi: 10.1155/2017/2974962. Epub 2017 Oct 18. PMID: 29234380; PMCID: PMC5664229.

Ventureyra, E. C. (2000). "Transcutaneous vagus nerve stimulation for partial onset seizure therapy. A new concept." Child's Nervous System 16(2): 101-102.

Howland, R. H. (2014). "Vagus Nerve Stimulation." Curr Behav Neurosci Rep 1(2): 64-73.

Dietrich, S., et al. (2008). "[A novel transcutaneous vagus nerve stimulation leads to brainstem and cerebral activations measured by functional MRI]." Biomedizinische Technik 53(3): 104-111.

Das, U. N. (2007). "Vagus nerve stimulation, depression, and inflammation." Neuropsychopharmacology 32(9): 2053-2054.

Miller, M. C. (2006). "What is vagus nerve stimulation?" Harvard Mental Health Letter 22(7): 8.

Buschman, H. P., et al. (2006). "Heart rate control via vagus nerve stimulation." Neuromodulation 9(3): 214-220.

Hirsch, E. F. (1962). "Innervation of the human heart. III. The conductive system." Archives of Pathology 74: 427-439.

Voss, A., et al. (2015). "Short-term heart rate variability--influence of gender and age in healthy subjects." PloS One 10(3): e0118308.

Mion, F., Pellissier, S., Garros, A., Damon, H., Roman, S., & Bonaz, B. (2020). Transcutaneous auricular vagus nerve stimulation for the treatment of irritable bowel syndrome: a pilot, open-label study. Bioelectronics in Medicine.doi:10.2217/bem-2020-0004

Grolaux P.J.D. (2019). Transcutaneous Vagus Nerve Stimulation in Private Healthcare Center: A Small-Scale Investigation Targeting Anxiety, Irritable Bowel Syndrome and Chronic Pain. J Neurol Neuromed 4(5): 7-22


  • NEIS_vagus-1

    Part 1 of Integrative Approach Targeting the NEI System and the Vagus Nerve

    Tax: +CA$105.00 HST+CA$20.13 service fee
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